Abstract:

An ion chromatographic method has been developed for the determination of sucrose, glucose and fructose in honey and wine. The optimal chromatographic conditions were investigated for the separation on METROSEP CARB 1 (150 mm × 4.0 mm) anion exchange column. Pulsed amperometric detector was used for detection. Elutions were performed using 10.0 mmol/L NaOH at a flow rate of 1.0 mL/min. The total analysis time was 25 min. The detection limits for sucrose, glucose, fructose were 0.085, 0.126 mg/L and 1.072 mg/L (20 μL injection), respectively. The RSDs of peak height for 8 consecutive determinations were between 3.12% and 10.56%, and the RSDs of retention time were between 2.36% and 3.25%. The spike recoveries ranged from 90.0% to 99.5%. This method is characteristic of simple sample pretreatment, high sensitivity and shorter analysis time, and can provide a simple and efficient method for the determination of honey and wine.

Key words: ion chromatography, pulsed amperometric detection, sucrose, glucose, fructose