FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (17): 14-17.doi: 10.7506/spkx1002-6630-201017003

• Basic Research • Previous Articles     Next Articles

Extraction Mechanism during the Sterilization of Supercritical Carbon Dioxide

ZHOU Xian-han1,2,CHENG Li-mei2,ZENG Qing-mei1,ZHANG An2,SONG Jun-hua2,ZHOU Dian-fei2   

  1. (1.Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230009, China;
    2.School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China)
  • Received:2009-11-09 Revised:2010-07-10 Online:2010-09-15 Published:2010-12-29
  • Contact: CHENG Li-mei2 E-mail:chenglimeicom@163.com

Abstract:

Functional components in cytoplasm, cell membrane and cell wall can be dissolved by supercritical carbon dioxide. As a result, the cells will be damaged and die due to inactivation. The extraction mechanism during supercritical carbon dioxide sterilization was explored in this study. An Escherichia coli suspension at a concentration of 108 CFU/mL was prepared and treated with supercritical carbon dioxide and composition analysis was performed on the extracts obtained at different time points. The results indicated that both non-polar fatty acids and polar weak small amino acids were the functional components in cells. This investigation demonstrates that extraction indeed occurs during supercritical carbon dioxide sterilization.

Key words: supercritical carbon dioxide, extraction, sterilization mechanism, Escherichia coli

CLC Number: