Optimization of Yeast Cell Disruption and Screening of Strains with High Peptidase Activity

doi:10.7506/spkx1002-6630-201017068

Abstract

Abstract:

To construct a method for determining total peptidase activity in yeast cells, a L9(34) orthogonal array design generated based on single-factor experiments was employed to optimize conditions for yeast cell disruption. In the optimization, the effects of 4 conditions for yeast cell disruption on total free amino acid amount in reaction system were dealt with. Snailase dose of 10 mg/mL, ultrasonic power of 500 W, ultrasonic treatment time of 8 min and ultrasonic operation interval of 5 s were found optimum. Under these optimum conditions, 61 yeast strains originated from unique Guizhou-style fermented soybean products were subjected to total peptidase activity determination, and 4 of them, namely WCF-5, XWF-1, XWF-7 and YZJ-4, were found have higher pepetidase activity.

Key words: yeast, disruption, total peptidases, fermented soybean products

CLC Number:

TS214.2

LI Yong-xia，ZENG Hai-ying，QIN Li-kang. Optimization of Yeast Cell Disruption and Screening of Strains with High Peptidase Activity[J]. FOOD SCIENCE, 2010, 31(17): 302-306.

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Optimization of Yeast Cell Disruption and Screening of Strains with High Peptidase Activity

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