FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (21): 236-238.doi: 10.7506/spkx1002-6630-201021053

• Bioengineering • Previous Articles     Next Articles

Sequence Analysis of Shiga Toxin Gene from Non-O157 Escherichia coli Strain Isolated from Foods

LI Rui1，DAI Shi-jiao1，DAI Kai1，DU De-long1，ZHU Ting-heng2,*   

  1. 1. College of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China；
    2. College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310000, China
  • Received:2010-01-11 Revised:2010-07-25 Online:2010-11-15 Published:2010-12-29
  • Contact: ZHU Ting-heng E-mail:thzhu@zjut.edu.cn

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Abstract:

A non-O157 E. coli strain (named EC6) which produces Shiga toxin type 1 was isolated from beef and the sequence of its Shiga toxin gene was analyzed. The full length of stx1 gene from strain EC6 was amplified by PCR, cloned into the pGEM-T Easy vector and sequenced. The results showed that the stx1 gene of EC6 had up to 99% similarity with the stx1 sequences deposited in GenBank database, indicating the occurrence of mutation. Furthermore, a phylogenetic tree was constructed by neighborjoining method, and according to it the stx1 gene of EC6 was stx1 genotype.

Key words: Shiga toxin-producing E. coli, food, stx1 gene, sequence analysis, non-O157 serotype

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