Abstract:

Objective: To construct a recombinant plasmid carrying extracellular protease gene from Lactobacillus. Methods: The extracellular protease gene was cloned from genomic DNA of L. helveticus and inserted into the expression vector pW425et. Then the recombination plasmid was transformed into the competence thyA gene-mutant E. coli X13. The expressed protein was analyzed by SDS-PAGE. Results: The recombinant shuttle plasmid pW425et-R was constructed successfully and the Lactobacillus extracellular proteinase gene was expressed in E. coli X13. Conclusion: Our research idea is feasible for the successful expression of Lactobacillus extracellular protease gene in E. coli X13, which will provide a basis for further preparation of recombinant Lactobacillus with anti-hypertensive function.

Key words: Lactobacillus, extracellular protease, anti-hypertensive peptide, cloning, expression