Abstract:

Objective: To investigate the hematopoiesis activity of bioactive peptides from Taihe Black-bone Silky Fowls (TBSF). Methods: Blood loss combined with myelosuppression drug (Fluorouracil, 5-FU) was used to establish a blood deficiency mouse model. Red blood cells (RBC) and hemoglobin (HGB) levels were measured to evaluate the hematopoiesis effects of bioactive peptides from TBSF and isolated bioactive peptide fraction A. Results: Compared with the blood deficiency group, the RBC level of bioactive peptides group has a significant difference on the 12th day administration (P＜ 0.05); During the period of 6 to 12 days, RBC and HGB levels of bioactive peptides group exhibited a significant faster than the Ejiao group (P＜0.05). On the 18th day, HGB level of bioactive peptides group was higher than other groups, and also significantly higher than the normal control group (P ＜ 0.05). The RBC level in bioactive peptide fraction A group was higher than the blood deficiency group at each time point although no significant difference was observed (P＞ 0.05), while the blood deficiency group and the Ejiao group exhibited a significant difference (P＜ 0.01), compared with the normal control group. On the 20th day, there was no significant difference in HGB level (P＞ 0.05) between the bioactive peptide fraction A group and the normal control group, while the blood deficiency group and the Ejiao group revealed a significant difference from the normal control group (P＜ 0.01). Conclusion: Bioactive peptides from TBSF exhibit hematopoiesis activity by elevating RBC and HGB levels to some extent.

Key words: Taihe black-bone silky fowl, bioactive peptide, blood deficiency model, hematopoiesis