Abstract: A HPLC method was developed for the simultaneous determination of aflatoxins B1, B2, G1 and G2 and ochratoxin A in cereal grains. Cereal grain samples were subject to extraction using methano1-water (80:20, V/V), liquid-liquid purification, enrichment and derivation using trifluoroacetic acid. The purification was performed on an Agilent ZORBAX SB-C18 column (4.6 mm × 250 mm) with a linear gradient elution of acetonitrile-2% glacial acetic acid. The detection limits of developed HPLC method for AFB1, AFB2, AFG1, AFG2 and OTA were 0.06, 0.03, 0.18, 0.05μg/kg and 0.51μg/kg, respectively. The linear detection ranges of developed HPLC method for AFB1, AFB2, AFG1, AFG2 and OTA was 0.05－100, 0.125－25.00, 0.05－100, 0.125 － 25.00μg/L and 0.05 － 50.00μg/L with correlation coefficients of 0.9998, 0.9998, 0.9998, 0.9996 and 0.9998, respectively. Recovery rates in cereal grains (corn, rice, wheat) spiked with mycotoxins were in the range of 71.73%－115.37% with the relative standard deviation of 3.00% － 9.88%. This HPLC method exhibited the capability to simultaneously determinate mycotoxins. Moreover, no significant difference was observed between the determination results and current national standard (P＞ 0.05).

Key words: high performance liquid chromatography, mycotoxins, simultaneous determination, cereal grain