Abstract:

Response surface analysis (RSA) methodology was used for optimizing homogenate extraction processing of arctiin from Arctium lappa L. Based on single-factor experiments, the effects of ethanol concentration, extraction time and liquid-material ratio on extraction rate of arctiin were explored by Box-Benhnken central composite design method. The optimal extraction conditions were ethanol concentration of 83%, extraction time of 93 s, liquid-material ratio of 17:1 (mL/g) and particle size of less than 1.0 mm. Under these optimal extraction conditions, the actual yield of total arctiin was 31.62 mg/g, which was consistent with the predicted yield of 31.72 mg/g. Results indicated that homogenate extraction was an effective method to extract arctiin from Arctium lappa L.

Key words: homogenate extraction, arctiin, response surface analysis (RSA)