FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (20): 221-225.doi: 10.7506/spkx1002-6630-201120046

• Analysis & Detection • Previous Articles     Next Articles

ESI-MS Analysis of Procyanidins from Sorghum Episperm and RP-HPLC-ESI-MS/MS Separation and Identification of Its Oligomers

XU Li-man,HUANG Man,TU Shi,CHEN Jing,LIU Rui*   

  1. (College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China)
  • Online:2011-10-25 Published:2011-10-12

Abstract: The composition of high-purity procyanidins purified by ADS-17 macroposours resin chromatography from sorghum episperm as a byproduct of sorghum processing was analyzed by electrospray ionization- mass spectroscopy (ESI-MS). Fraction SPC-5 separated from the procyanidins by Toyopearl HW-40 resin chromatography was identified by RP-HPLC-ESI-MS/MS. The high-purity procyanidins were found to be procyanidolic oligomers, including catechin/epicatechin monomers, dimmers, trimers, tetrames, pentamers and hexamers. SPC-5 consisted of 8 trimers and 1 dimmer.

Key words: sorghum procyanidins, gel chromatography, electrospray ionization-mass spectroscopy (ESI-MS),
reverse phase high-performance liquid chromatography-mass spectroscopy/mass spectroscopy (RP-HPLC-ESI- MS/MS),
identification

CLC Number: