in vitro Conversion of β-Carotene Produced by Rhodotorula into Vitamin A

doi:10.7506/spkx1002-6630-201123040

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (23): 201-205.doi: 10.7506/spkx1002-6630-201123040

• Bioengineering • Previous Articles Next Articles

in vitro Conversion of β-Carotene Produced by Rhodotorula into Vitamin A

LIU Hui-lin1，LIU Shao1,2，XIE Da-ping3,*

(1. College of Bio-Safety Science and Technology, Hunan Agricultural University, Changsha 410128, China； 2. College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China； 3. College of Bio-Science and Technology, Hunan Agricultural University, Changsha 410128, China)

Online:2011-12-15 Published:2011-12-21

Abstract

Abstract: The existence ofβ-carotene in the fermentation broth of Rhodotorula was identified by HPLC, and its in vitro cleavage into retinol also known as vitamin A byβ-carotene 15,15＇-monooxygenase from chicken intestinal mucosa was studied. The optimum reaction conditions for converting 123 mg/Lβ-carotene concentrate were determined to be: pH 8.0, 3.5 mmol/L deoxycholate, 0.5 mmol/L d-α-tocopherol and 0.25 g/100 mL Tween-40. Under the optimum conditions, 7 h incubation of 2.58 nmol/(mg ·h )β-carotene 15,15＇-monooxygenase in 37 ℃ water bath with shaking produced 40.1 mg/L retinol, with a conversion yield of 32.6%.

Key words: Rhodotorula, β-carotene, β-carotene-15,15O`-monooxygenase, retinol, reaction conditions optimization

CLC Number:

R151.2

LIUHui-lin，LIUShao，XIEDa-ping. in vitro Conversion of β-Carotene Produced by Rhodotorula into Vitamin A[J]. FOOD SCIENCE, 2011, 32(23): 201-205.

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in vitro Conversion of β-Carotene Produced by Rhodotorula into Vitamin A

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