Induced Mutation Breeding and Screening of a Coenzyme Q10-Producing Strain

doi:10.7506/spkx1002-6630-201211027

Abstract

Abstract: Rhizobium leguminosarum 1.1723 was mutagenized by means of LiCl treatment followed by UV irradiation or ultrasonic treatment alone to yield coenzyme Q10-producing strains. The results showed that 1 g/L LiCl could obviously increase the positive mutation rate and that ultrasonic treatment could change cell wall structure of Rhizobium leguminosarum. A mutant C40-05 with high productivity of coenzyme Q10 was obtained through preliminary screening with roxithromycin and secondary screening by means of shake flask cultivation. Its coenzyme Q10 yield was up to 1.198 mg/g, which revealed a 208% increase compared with the original stain. Only 2.67% reduction in coenzyme Q10 yield of the mutant was observed after the fifth passage. Therefore, the obtained mutant is a stable strain that deserves to be studied further.

Key words: Rhizobium leguminosarum, coenzyme Q10, UV-LiCl, ultrasonic, mutagenesis

CLC Number:

TS201.3

CAOYan-ni，YUETian-li，YUANYa-hong，GAOZhen-peng. Induced Mutation Breeding and Screening of a Coenzyme Q10-Producing Strain[J]. FOOD SCIENCE, 2012, 33(11): 121-125.

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Induced Mutation Breeding and Screening of a Coenzyme Q10-Producing Strain

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