Identification of Unknown Pigment in Inferior Black Sesame Paste

doi:10.7506/spkx1002-6630-201212040

FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (12): 200-204.doi: 10.7506/spkx1002-6630-201212040

• Analysis & Detection • Previous Articles Next Articles

Identification of Unknown Pigment in Inferior Black Sesame Paste

DING Hua1，DONG Li-na2，CHEN Jian-biao2，YANG Jie1，XIA Hong1，PENG Mao-min1， WANG Qing-qing1，HU Ding-jin1，ZHOU You-xiang1,*

(1. Institute of Quality Standard and Testing Technology for Agro-Products, Hubei Academy of Agricultural Sciences, Wuhan 430064, China；2. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China)

Online:2012-06-25 Published:2012-07-27

Abstract

Abstract: An unknown blue pigment was extracted from an inferior black sesame paste on the market. The maximum absorption wavelength of the pigment was determined by UV-visible spectroscopy. It was analyzed qualitatively by HPLC-MS and analyzed quantitatively by HPLC. The pigment had a high solubility in methanol. It could be effectively separated on Inertsil ODS-3 column using a mobile phase composed of 20 mmol/L ammonium acetate solution and acetonitrile (V/V = 70:30). It was identified as brilliant blue through ion-trap MS analysis. The results of quantitative analysis by HPLC with diode-array detection indicated that the content of brilliant blue in the inferior black sesame paste was 1.73 mg/kg.

Key words: food authenticity, sesame paste, brilliant blue, MS analysis

CLC Number:

O656.22

DING Hua，DONG Li-na，CHEN Jian-biao，YANG Jie，XIA Hong，PENG Mao-min，WANG Qing-qing，HU Ding-jin，ZHOU You-xiang. Identification of Unknown Pigment in Inferior Black Sesame Paste[J]. FOOD SCIENCE, 2012, 33(12): 200-204.

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Identification of Unknown Pigment in Inferior Black Sesame Paste

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