Determination of Brilliant Blue Isomers in Food by High Performance Liquid Chromatography

doi:10.7506/spkx1002-6630-201208048

FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (8): 221-224.doi: 10.7506/spkx1002-6630-201208048

• Analysis & Detection • Previous Articles Next Articles

Determination of Brilliant Blue Isomers in Food by High Performance Liquid Chromatography

LIU Liping1，HUANG Wei2 ，TANG Xu-wen1，PENG Gao-ying1，LIU Guo1，CHEN Lin-qiao1

(1. Institute of Product and Commodity Quality Inspection and Supervision, Huaihua Quality and Technical Supervision Bureau, Huaihua 418000, China；2. Institute of Quality Inspection and Measurement Calibration, Zhongfang County Bureau of Quality and Technical Supervision, Zhongfang 418005, China)

Online:2012-04-25 Published:2012-03-31

Abstract

Abstract: A new analytical method was developed for the determination of brilliant blue in food by high performance liquid chromatography (HPLC). It was preliminarily confirmed that the presence of brilliant blue in food was mixtures of its three isomers. Sample extracts were purified by polyamide column chromatography and separated on an Inertsil ODS-SP C18 (150 mm × 4.6 mm, 5 μm) column using a mobile phase made up of methanol and 0.02 mol/L ammonium acetate solution. The detection wavelength was set as 629 nm. The spike recovery rates across three spike levels were in the range of 91.1%－94.7%. The method was simple and accurate.

Key words: brilliant blue isomers, high performance liquid chromatography (HPLC), food

CLC Number:

O658

LIULiping，HUANGWei，TANGXu-wen，PENGGao-ying，LIUGuo，CHENLin-qiao. Determination of Brilliant Blue Isomers in Food by High Performance Liquid Chromatography[J]. FOOD SCIENCE, 2012, 33(8): 221-224.

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Determination of Brilliant Blue Isomers in Food by High Performance Liquid Chromatography

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