Isolation, Identification and Carbon Metabolic Fingerprinting Analysis of Four Pathogens Isolated from Postharvest Plum Fruit

doi:10.7506/spkx1002-6630-201213050

FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (13): 235-239.doi: 10.7506/spkx1002-6630-201213050

• Bioengineering • Previous Articles Next Articles

Isolation, Identification and Carbon Metabolic Fingerprinting Analysis of Four Pathogens Isolated from Postharvest Plum Fruit

WANGYou-sheng，CHENYu-juan，ZHANGYan

(Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, Beijing Key Laboratory of Food Flavor Chemistry, School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China)

Online:2012-07-15 Published:2012-07-27

Abstract

Abstract: Four pathogens, strain 059#, 067#, 087# and 088#, were isolated from infected plum fruit during postharvest storage. Morphological characterization and rDNA ITS analysis confirmed that the four pathogens were Gibberella moniliformis, Alternaria alternate, Penicillium oxalicum and Aspergillus tubingensis, respectively. A Biolog Microbial Identification System with FF MicroPlate was applied for carbon source utilization tests of the four pathogens based on their abilities to utilize 95 different substrates. The results of carbon metabolic fingerprinting analysis showed that all the isolated pathogens had more than 77 optimal carbon sources, 45 ones in common such as D-fructose, D-glucosamine, sucrose, sorbitol, malic acid and citric acid.

Key words: plum fruit, fungal pathogen, ITS rDNA sequence analysis, carbon metabolic fingerprinting

CLC Number:

TS207.3

WANGYou-sheng，CHENYu-juan，ZHANGYan. Isolation, Identification and Carbon Metabolic Fingerprinting Analysis of Four Pathogens Isolated from Postharvest Plum Fruit[J]. FOOD SCIENCE, 2012, 33(13): 235-239.

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Isolation, Identification and Carbon Metabolic Fingerprinting Analysis of Four Pathogens Isolated from Postharvest Plum Fruit

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