Identification of Hafnia alvei and Its Ability to Produce Biogenic Amine

doi:10.7506/spkx1002-6630-201213049

Abstract

Abstract: A biogenic amine-producing strain named as C10 was isolated from chilled chicken in our laboratory. Its effect on the safety of chilled chicken was evaluated during storage. The strain was identified according to its physiological and biochemical characteristics and 16 rDNA sequence. At the same time, its ability to produce biogenic amine during culture in decarboxylase medium at 4 ℃ for 3 days was analyzed by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). The strain C10 was identified as Hafnia alvei. Consistent results were obtained using TLC and HPLC, which showed that C10 could produce cadaverine, putrescine and histamine at levels of 12.28, 5.23 mg/L and 8.35 mg/L, respectively. In conclusion, Hafnia alvei is a potential hazard to the safety of chicken during refrigeration. In addition, TLC is proved to be a rapid, economical and reliable method for detecting BAs in meats.

Key words: biogenic amines, 16S rRNA, Biolog analysis, thin layer chromatography(TLC), high performance liquid

CLC Number:

Q939.97

WANGMiao，DINGWen，WANGXian-ding，ZHULiang，SUNQun. Identification of Hafnia alvei and Its Ability to Produce Biogenic Amine[J]. FOOD SCIENCE, 2012, 33(13): 230-234.

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Identification of Hafnia alvei and Its Ability to Produce Biogenic Amine

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