FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (13): 26-30.doi: 10.7506/spkx1002-6630-201213003

• Basic Research • Previous Articles     Next Articles

Spectroscopic Properties of in vivo and in vitro Digestive Resistant Starch from High-Amylose Transgenic Rice

MANJian-min,ZHOUXing-hua,DONGYing,LIUQiao-quan,WEICun-xu   

  1. (1. Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Key Laboratory of Plant Functional Genomics, Ministry of Education, Yangzhou University, Yangzhou 225009, China; 2. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China)
  • Online:2012-07-15 Published:2012-07-27

Abstract: In this study, the spectroscopic properties of in vivo and in vitro digestive resistant starches from a high-amylose transgenic rice line (TRS) established through antisense RNA inhibition of starch branching enzymes were investigated by X-ray powder diffraction and Fourier transform infrared spectroscopy. The results showed that the amylose contents of non-digestive TRS resistant starch significantly increased. TRS starch was C-type crystalline starch, a mixture of A- and B-type crystalline starches. The degradation of the A-type crystalline starch was faster than that of B-type starch, and the B-type crystalline starch showed a high resistance to digestion. The amorphous starch of starch granule was easy to be degraded. These data could be useful for various applications of high-amylose crop in the food and nonfood industry.

Key words: rice, high-amylose starch, in vivo digestion, in vitro digestion, spectroscopic property

CLC Number: