Effect of Supercritical Carbon Dioxide Density on Absorption Coefficient of All-trans Lycopene

Abstract

Abstract: Objective: To assess the effect of varying carbon dioxide density on absorption coefficient (A1cm) of all-trans lycopene through exploring the correlation between carbon dioxide density in supercritical fluid chromatography (SFC) and peak areas of all-trans lycopene fractions. Methods: Supercritical CO2 fluid chromatography was carried out on a Diamonsil C8 (250mm × 4.6 mm, 5 μm, ) at a pressure ranging from 11.5 to 17.5 MPa, and a temperature ranging from 35—55 ℃. The detection wavelength was 453 nm, and 20 μL sample was injected for SFC chromatography. Results: Peak area of all-trans lycopene at the maximum absorption wavelength in supercritical carbon dioxide fluid chromatography revealed a variation and was linearly positive correlated with the density of carbon dioxide. Conclusion: Absorption coefficient of all-trans lycopene is positively correlated with the density of supercritical carbon dioxide.

Key words: all-trans lycopene, supercritical fluid chromatography (SFC), supercritical carbon dioxide, density, absorption coefficient (A1cm)

References

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