FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (16): 175-178.

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Comparative Analysis of Bioactive Component in Seeds and Flesh of Schisandrae chinensis Fructus by Thin Layer Chromatography

  

  • Received:2011-07-29 Revised:2012-07-08 Online:2012-08-25 Published:2012-09-07
  • Contact: SHI Wei-Jing E-mail:shiwj80@yahoo.com.cn

Abstract: Objective: To comparatively analyze bioactive components in seeds and flesh of Schisandrae chinensis Fructus. Methods: The contents of lignan in seeds, flesh and whole fruit of Schisandrae chinensis Fructus were determined by high performance thin layer chromatography (HPTLC). Sulfuric acid-phenol method was used to quantify sugar. After trifluoroacetic acid hydrolysis, the monosaccharide composition of the flesh was analyzed by HPTLC. Results: Lignans in Schisandrae chinensis Fructus were mainly present in the seeds, and total sugar content was 169.34 mg/g in the flesh and 5.366 mg/g in the seeds. The alcohol-soluble, alcohol-insoluble and polysaccharide components were 117.06, 75.67 mg/g and 12.37 mg/g in the fresh, respectively. The alcohol-soluble fraction was mainly composed of glucose. The polysaccharide fraction was mainly composed of glucose and galactose. Conclusion: Bioactive components reveal a significant difference between seeds and flesh of Schisandrae chinensis Fructus. 

Key words: Schisandrae chinensis Fructus, seeds, flesh, lignanoid, polysaccharides

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