Optimization of Fermentation Conditions for the Production of Glycerol from Saccharomyces cerevisiae

Abstract

Abstract: In order to improve the production of glycerol from Saccharomyces cerevisiae D254, the effect of fermentation conditions such as initial glucose or fructose content, pH, temperature and SO2 amount on the yield of glycerol were explored by one-factor-at-a-time and Plackett-Burman array design methods. The optimal fermentation conditions for the production of glycerol were 216 g/L initial glucose, 144 g/L fructose, fermentation pH 3.0, fermentation temperature 32 ℃ and 40 mg/L SO2. Under these conditions, the highest yield of glycerol was achieved, 655.64 μmol/L.

Key words: Saccharomyces cerevisiae, fermentation conditions, glycerol yield

CLC Number:

TS261.1

References

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