RAPD Typing of Enterohemorrhagic E. coli Mutants

FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (17): 184-187.

RAPD Typing of Enterohemorrhagic E. coli Mutants

Received:2011-07-25 Revised:2012-08-02 Online:2012-09-15 Published:2012-11-09
Contact: Rui Li E-mail:liruiwuhan77@yahoo.com.cn

Abstract

Abstract: Four enterohemorrhagic Escherichia coli (EHEC) strains were analyzed by random amplified polymorphic DNA (RAPD) method, and the reliability of genotyping of pathogenic E. coli by RAPD was discussed in terms of the major toxic genes, eae and hly and Shiga toxin titer. Two of the test strains carried stx genes but did not express Shiga toxins. Of them, one mutant strain, O157 EC169, had a similar RAPD profile with the other two O157 strains which could express Shiga toxins, while the other mutant, O157 EC130 revealed significant cluster difference from other strains. Several primers with strong repeatability and polymorphism that are available for fast identification of E. coli pathogenic bacteria and the food poisoning traceability were selected from 20 random primers.

Key words: enterohemorrhagic Escherichia coli (EHEC) , Shiga toxins, random amplified polymorphic DNA (RAPD) , genotyping

CLC Number:

R155.5

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