FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (20 ): 272-276.doi: 10.7506/spkx1002-6300-200920058

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Detection of Genetic Diversity of Agaricus bisporus Using RAPD Markers

LIN Yuan1,CHEN Wen-bing2,*,SHAO Bi-ying2,WANG Ze-sheng3,LIAO Jian-hua3,LI Shou-song2,JIANG Shu-xun2   

  1. 1. Jinshan College, Fujian Agriculture and Forestry University, Fuzhou 350002, China ;2. Fujian Entry-Exit Inspection and
    Quarantine Bureau, Fuzhou 350001, China ;3. Fujian Academy of Agricultural Sciences, Fuzhou 350014, China
  • Received:2009-07-07 Online:2009-10-15 Published:2010-12-29
  • Contact: CHEN Wen-bing2,*, E-mail:621213wbc@163.com

Abstract:

The genetic diversity of 40 strains of Agaricus bisporus was assessed using RAPD markers. Of 108 random primers, 71 were informative and yielded 567 bands, 434 of which were polymorphic with a mean of 7.99 bands per primer. Cluster analysis was performed by between-groups linkage method and coefficient of euclidean distance square (CEDS) method using SPSS 11.0. As a result, these Agaricus bisporus strains could be clustered into 3 groups at 20.5 in CEDS, or could be clustered into 6 groups at 8.5 in CEDS, which is in considerable agreement with the results obtained by isoenzyme clustering.

Key words: Agaricus bisporus, random amplified polymorphic DNA (RAPD), genetic diversity, cluster analysis, genetic relationship

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