Separation, Purification and Anti-Aging Activity of Phycocyanin from Porphyra yezoensis

Abstract

Abstract: Objective: To isolate and purify phycocyanin from Porphyra yezoensis and explore the anti-aging activity of phycocyanin. Methods: Phycocyanin from Porphyra yezoensis was purified by salting-out and Sephadex G200 chromatography. The stability of phycocyanin under various pH and temperature conditions was also investigated. D-Galactose induced mouse models of subacute ageing were established and used to study the anti-ageing function of phycocyanin from Porphyra yezoensis. Results: Phycocyanin with the highest purity (1.73) was obtained by salting out with 40% (NH4)2SO4 at room temperature. The hycocyanin extract was stable at 30 ℃ and pH 7.0. Its oral administration resulted in a significant increased in SOD activity, thymus index and spleen index in aging mouse models (P＜0.05, P＜0.01), and a significant reduction in serum MDA level (P＜0.05, P＜0.01). Conclusion: Phycocyanin from Porphyra yezoensis has excellent anti-aging activity.

Key words: Porphyra yezoensis, phycocyanin, stability, anti-aging, thymus index, spleen index

CLC Number:

中图分类号：TS202.3

References

[1] 曾呈奎，王素娟，刘思俭等，海藻栽培学[M]．上海：上海科学技术出版社1984:135-137．
[2] 任姗姗，李鹏高，张淑华等，条斑紫菜酶解多肽的保健功能作用食品研究与开发[J]．2011，32(2)：38-41
[3] WANG C, DONG D, WANG G C, et al. An improved PCR method for direct identification of Porphyra (Bangiales, Rhodophyta) using conchocelis based on a RUBISCO intergenic spacer [J]. Chinese Journal of Oceanology and Limnology 2009, 27(3):513-518
[4] 李博文，左正宏，董宏坡等，坛紫菜别藻蓝蛋白的纯化及多克隆抗体的制备高技术通讯[J]．2006，16(6)：638-642
[5] 蔡春尔，周铭，李春霞等，条斑紫菜藻胆蛋白提纯方法优化探索[J]．生物技术通报，2009，2：98-102
[6] 左正宏，邓元告，陈奕欣等，坛紫菜别藻蓝蛋白α,β亚基基因的克隆和序列分析[J]．海洋学报，2005，27(2)：148-152
[7] 成华，向文洲，吴华莲等，海水螺旋藻C一藻蓝蛋白富硒及其抗氧化特性[J]．热带海洋学报，2008，27(5)：60-63
[8] 刘杨，王雪青，庞广昌等. 钝顶螺旋藻藻蓝蛋白的富集分离及其稳定性研究[J]．食品科学，2008，29(7)：39-42
[9] 汪兴平，谢笔均，潘思轶等，葛仙米藻蓝蛋白抗氧化作用研究[J]．食品科学，2007，8(12)：458-461
[10] Bhat V B，Madyastha K M．C-Phycocyanin：A potent Peroxyl Radical Scfivenger in vivo and in vitro[J]．Biochem Biophys Res Commun, 2000, 275: 20-25
[11] Romay C，Gonzdlez R，Led6n N，et a1．C-Phycocyanin：A biliprotein with Antioxidant，Anti-Inflammatory and Neuroprotective effects[J]．Current Protein Peptide Sci，2003, 4: 207-216
[12] 周站平，陈秀兰，陈超等，藻胆蛋白脱辅基蛋白对其抗氧化活性的影响[J]．海洋科学，2003，27(5)：77-81
[13] Remirez D, Fernandez V, Tapia G, et a1. Influence of C phycocyanin on hepatoeellular parameters related to liver oxidative stress and Kupffer cell functioning[J]．Inflamm Ras, 2002, 5l: 35l -356
[14] 郝玮，潘岭．螺旋藻藻蓝蛋白生理活性的研究进展[J]．医学综述，2006，12(2)：111-113
[15] Romay C，Delgado R，Remirez D，et a1．Effects of phycocyanin extract on tumor necrosis factor-alpha and nitrite levels in serum of mice treated with endo-toxin[J]．Rzneimittelforschung，2001, 51: 733-736
[16] Junjaud G，Rouaud E，Turpin F，et a1．Age-related effects of the neuromodulator
D-serine on neurotransmission and synaptic potentiation in the CA I hippocampal area of the rat [J]．J Neurochem, 2006, 98(4): l159-1163．
[17] 王广策，曾呈奎，钝顶螺旋藻C-藻蓝蛋白分子内不同基团间能量传递的研究[J]．海洋与湖沼，1998，9(5)：471-476