Optimization of Microwave-Assisted Extraction of Flavonoids from Propolis by Response Surface Methodology

Abstract

Abstract: This paper reports the optimization of process conditions for microwave-assisted extraction of flavonoids from propolis using response surface methodology. The effects of six operating parameters including microwave treatment time, microwave power, solvent composition (ethanol concentration), temperature, time and solvent-to-solid on total flavonoid yield were investigated through one-factor-at-a-time experiments. The significance and interaction effects of microwave treatment time, microwave power, ethanol concentration and temperature were analyzed by response surface analysis based on a three-level central composite design. The optimal microwave-assisted extraction conditions for total flavonoids were determined as follows: 70 s microwave treatment at 282 W followed by extraction with 80% ethanol aqueous solution at a solvent-to-solid of 25 mL/g and 77 ℃ for 12 h. Under these conditions, the yield of total flavonoids from propolis was up to 25.08%.

Key words: propolis, flavonoids, extraction, response surface methodology

References

[ ] 杨兆起,封秀娥.中药鉴别手册(第三册) [M].北京:科学出版社,1994 102.
[ ] 张源源,崔同.总黄酮含量分析法快速评价蜂胶原料质量[J].中国食品学报,2002,2(3):46.
[ ] Beatriz C.B.S. Mello, José Carlos Cunha Petrus, Miriam Dupas Hubinger.Concentration of flavonoids and phenolic compounds in aqueous and ethanolic propolis extracts through nanofiltration. Journal of Food Engineering 96 (2010) 533–539
[ ] 王贻节.蜜蜂产品学[M].北京:农业出版社,1995,83-89.
[ ] M. Blonska, J. Bronikowska, G. Pietsz, Z.P. Czuba, S. Scheller, W. Krol .Effects of ethanol extract of propolis (EEP) and its flavones on inducible gene expression in J774A.1 macrophages. Journal of Ethnopharmacology 91 (2004) 25–30.
[ ] 程伟贤,陈鸿雁,张义平等.蜂胶中黄酮成分及提取方法研究[J].云南大学学报(自然科学版),2006,28(S1):245-147
[ ] Chao-Rui Chen , Ying-Nong Lee , Chieh-Ming J. Chang et al. Hot-pressurized fluid extraction of flavonoids and phenolic acids from Brazilian propolis and their cytotoxic assay in vitro. Journal of the Chinese Institute of Chemical Engineers 38(2007) 191–196
[ ] 刘元法,王兴国,金青哲.超声波技术提取蜂胶黄酮类功能性物质的研究[J].食品科学,2004,25(6)35-39
[ ] 牟兰,王海燕,曾唏.贵州蜂胶成分分析(二) [J]中国养蜂,2001,52(2):12-13.
[ ] 王影.绿豆牙总黄铜的提取工艺研究[J].食品与发酵科技,2010,46(5)45-48.
[ ] 吴东青,徐新建等.响应面分析法优化竹节草黄酮提取工艺[J]食品科学,2011,32(8):125-128.
[ ] 刘洋，赵谋明，杨宁．响应面分析法优化仙人掌多糖提取工艺的研究[J].食品与机械,2006，22(6)：42-45.
[ ] 姚狄,马平,王颖等.响应面法优化玉米芯中木聚糖的提取工艺[J].食品科学,2011,32(8):111-115.
[ ] 杨文雄,高彦祥.响应面法及其在食品工业中的应用[J].中国食品添加剂,2005(2): 68-71.
[ ] 李亚娜,林永成,佘志刚.响应面分析法优化羊栖菜多糖的提取工艺[J].华南理工大学学报:自然科学版，2004,32(11):29-31.

[1]	WANG Feng, XIAO Chuxiang, LIU Shuzhen, WANG Fengwu. Extraction of Flavonoids from Durian Seeds and Its Antioxidant and Anti-aging Effects in Caenorhabditis elegans [J]. FOOD SCIENCE, 2021, 42(9): 123-129.
[2]	LIU Panpan, ZHENG Pengcheng, GONG Ziming, CHANG Zerui, SU Fangjun, HUANG Bo, FENG Lin, GAO Shiwei, ZHENG Lin. Analysis of Aroma Characteristics and Volatile Components of Juhong Tea, Manufactured from Black Tea with Added Citrus Peel [J]. FOOD SCIENCE, 2021, 42(8): 198-205.
[3]	LI Kailong, CHEN Tongqiang, XU Wenyang, LI Tao, WANG Liangliang, WANG Fang, SUN Guifang. Ultrasonic Assisted Cloud Point Extraction Combined with Liquid Chromatography-Tandem Mass Spectrometry for the Determination of Natural Vitamin K Homologues in Vegetables [J]. FOOD SCIENCE, 2021, 42(8): 214-220.
[4]	WANG Suli, GUO Zhenfu, GENG Lili. Liquid Phase Microextraction with Deep Eutectic Solvent Combined with High Performance Liquid Chromatography for Determination of New Neonicotinoid Insecticide Residues in Edible Oil [J]. FOOD SCIENCE, 2021, 42(8): 277-282.
[5]	ZHANG Qiyue, ZHANG Shikai, XI Liangqing, DU Haiyun, WU Peng. Effects of Different Extraction Methods on the Structure, Physicochemical and Functional Properties of Water-Soluble Dietary Fiber from Cherry Wine Dregs [J]. FOOD SCIENCE, 2021, 42(7): 98-105.
[6]	MENG Zhijuan, HUANG Yunxia, LI Yan, SUN Wenyi, WANG Dong, LI Qiang, FAN Sufang, ZHANG Yan. Rapid Determination of 17 Phthalate Acid Esters Residues in Vegetable Oils by Gas Chromatography-Electrostatic Field Orbitrap High Resolution Mass Spectrometry [J]. FOOD SCIENCE, 2021, 42(6): 298-304.
[7]	CHU Yaojuan, ZHANG Xuena, XIANG Xiaozhe, LI Xiujuan. Determination of Multiple Organophosphorus Pesticide Residues in Fruits and Vegetables by Molecularly Imprinted Array Solid Phase Microextraction [J]. FOOD SCIENCE, 2021, 42(6): 310-315.
[8]	HE Wenjie, WU Binbin, XU Wei, WANG Hongxun, YI Yang, LEI Feifei. Effect of Flavonoids from Gordon Euryale Seeds on the Structure of Heat-treated Myofibrillar Proteins [J]. FOOD SCIENCE, 2021, 42(6): 46-52.
[9]	CHENG Chao, XIA Lanxin, DU Fenni, LI Wei, TIAN Cheng. Optimization of Solid Phase Microextraction for GC-MS Analysis of Nine Flavor Compounds in Lichuan Hong Tea and Flavor Discrimination by Partial Least Squares-Discriminant Analysis [J]. FOOD SCIENCE, 2021, 42(6): 215-222.
[10]	WANG Xiangxin, SHAN Yi, WEI Xuedong, LI Wenbin, E Laiming, JIANG Yujun. Determination of Nucleotides in Infant Formula by Ultra-high Performance Liquid Chromatography [J]. FOOD SCIENCE, 2021, 42(4): 254-262.
[11]	PENG Changmei, FANG Ruilin, LAI Min, DENG Zeyuan, LIU Xiaoru, LI Jing. Effects of Different Extraction Methods on the Quality of Peony (Paeonia suffruticosa Andr.) Seed Oil [J]. FOOD SCIENCE, 2021, 42(3): 104-111.
[12]	LIU Meng, GAO Haiyan, FANG Xiangjun, WU Weijie, CHEN Hangjun, LIU Ruiling. Optimization of Ultrasonic-Microwave Assisted Extraction of Phenolic Acids from Chinese Bayberries (Morella rubra Sieb. et Zucc) of Different Maturities and a Comparative Study of Their Antioxidant Activities [J]. FOOD SCIENCE, 2021, 42(3): 112-120.
[13]	MA Yongqiang, XIU Weiye, LI Chenchen, WANG Yiqi, CHEN Junjie. Optimization of Preparation Process for Lycopene-Loaded Nanostructured Lipid Carrier by Central Composite Design-Response Surface Methodology [J]. FOOD SCIENCE, 2021, 42(3): 121-127.
[14]	ZHOU Nong, YU Zhiliang, LI Chengyong. Fabrication and Antioxidant Activity of Zein-Guava Flavonoid Composite Nanoparticles [J]. FOOD SCIENCE, 2021, 42(3): 186-193.
[15]	DAI Jinbo, SHEN Jie, HE Xiaofeng, NIE Rongrong, DONG Wenjing, LIANG Qinxian. Optimized QuEChERS Combined with UPLC-MS/MS for Determination of Fipronil and Its Metabolites in Poultry-Derived Foods [J]. FOOD SCIENCE, 2021, 42(2): 325-332.