Abstract: The composition and structure of glycerides in chicken fat were analyzed by reversed-phase high performance liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (RP-HPLC-APCI MS) and reversed-phase high performance liquid chromatography with an evaporative light-scattering detector (RP-HPLC-ELSD). The chromatographic separation was performed on a C18 using a mobile phase of acetonitrile-methylene chloride (60:40, V/V). Glycerides were monitored by APCI MS in positive mode. According to [M+H]＋ and [M+H-RCOOH]＋, 26 glycerides were identified. Based on the abundance of [M+H-RCOOH]＋ and ion fragments from collision induced dissociation (CID) of ion trap, the distribution of fatty acids in glycerides was deduced. Triacylglycerols represented 99.88% of total glycerides as demonstrated through RP-HPLC-ELSD analysis and quantification by peak area normalization. Among these triacylglycerols, 1-palmitoyl-2,3-di-oleoyl-glycerol revealed the highest content (23.65%), followed by 1-palmitoyl-2-oleoyl-3-linoleoyl-glycerol (19.37%), and 1-linoleoyl-2,3-di-oleoyl-glycerol (16.02%).

Key words: chicken fat, glyceride, RP-HPLC, APCI MS, ELSD