Purification and Structural Characterization of Exopolysaccharide Produced by Streptococcus thermophilus CH9

Abstract

Abstract: Crude exopolysaccharides (EPS) isolated from the fermentation supernatant of Streptococcus thermophilus CH9 were fractionated by DEAE-52 column chromatography into EPS1, EPS2 and EPS3. Sephadex G-100 column chromatography was used to further fractionate the three factions to harvest their respective major components named as EPS1a, EPS2a and EPS3a, respectively. EPS1a was a neutral polysaccharide with a molecular weight of 1.8 × 106 u, and EPS2a and EPS3a were acidic polysaccharides with a molecular weight of 1.06 × 106 u and 1.05 × 106 u, respectively. Agarose gel electrophoresis analysis indicated that all these three polysaccharides consisted of a single component. UV and IR spectroscopic analyses showed that all of them had characteristic adsorption peaks of polysaccharide. EPS2a and EPS3a might be polysaccharide-protein complexes. Congo red test revealed that EPS1a rather than EPS2a and EPS3a had a three-stranded helix structure. Beta elimination test demonstrated that EPS1a and EPS3a were -O-linked, but EPS2a was a -N-linked polysaccharide.

Key words: Streptococcus thermophilus CH9, exopolysaccharides, purification, structural characterization

CLC Number:

TS252

Xin-Yuan ZHANG. Purification and Structural Characterization of Exopolysaccharide Produced by Streptococcus thermophilus CH9[J]. FOOD SCIENCE, 2012, 33(19): 107-112.

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