Mechanism Underlying the Inactivation of E. coli by High Pressure Carbon Dioxide (HPCD)

Abstract

Abstract: The efficacies of high pressure carbon dioxide (HPCD) treatment for inactivating dried culture powder and suspension of E. coli were comparatively evaluated by measuring the activity of H+-ATPase in E. coli, determining the amino acid composition of HECD extracts using an amino acid analyzer, and observing the morphology of E. coli cells under a scanning electron microscope (SEM) before and after HPCD treatment. The results obtained demonstrated that the inactivation of E. coli by HPCD was achieved jointly by mechanical rupture, extraction and acidification rather than a single action.

Key words: high pressure carbon dioxide (HPCD), E. coli, inactivation mechanism, acidification, extraction, rupture

CLC Number:

TS201.3

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