Abstract: Goldfishes (Carassius auratus) were exposed to rhodamine B at concentrations of 5 mg/L and 15 mg/L in water to explore the accumulation of rhodamine B in the liver, kidney, cheek, muscle and spine. Meanwhile, additional goldfishes were intraperitoneally injected with rhodamine B and placed back into dechlorinated tap water to investigate the elimination of rhodamine B. Rhodamine B reached an equilibrium state in goldfishes after 15 days and 12 days of absorption at 5 mg/L and 15 mg/L. The maximum and minimum accumulation coefficients of rhodamine B for equilibrium absorption at the high and low concentrations were observed in the liver and muscle, respectively, which were 16.14 and 7.37 for maximum accumulation, and were 0.97 and 0.77 for minimum accumulation, respectively. The concentrations of rhodamine B in goldfish organs were gradually eliminated and reached equilibrium on the 9th day after intraperitoneal injection. Different goldfish tissues could be ranked as follows: kidney ＞ muscle ＞ bone ＞ gill ＞ liver in decreasing order of the elimination rate of rhodamine B, and liver ＞ gill ＞ bone ＞ kidney ＞ muscle in decreasing order of the half-life of rhodamine B.

Key words: rhodamine B, Carassius auratus, accumulation, elimination, bioaccumulation coefficient