Identification and Antibacterial Components of Bacterial Strain F35 Isolated from Pinctada fucata Mucus

Abstract

Abstract: Bacterial strain F35, isolated from Pinctada fucata mucus using Zobell 2216E medium, had an inhibitory effect on six common foodborne pathogens. The strain was identified as Bacillus methylotrophicus based on its morphological, physiological and biochemical characteristics and 16S rRNA sequence. After removal of acids and hydrogen peroxide, its fermentation supernatant was treated separately with four proteases. Compared with the fermentation supernatant, all the resulting hydrolysats showed a decrease in inhibitory effect against Listeria monocytogenes. In conclusion, the antibacterial substances of strain F35 are protein or polypeptide and thus have great potential to be developed as new natural food preservatives.

Key words: Pinctada fucata mucus, bacteria F35, identification, antibacterial component

CLC Number:

Q 939.11+7

WU Yan-Yan Laihao LI Xianqing YANG. Identification and Antibacterial Components of Bacterial Strain F35 Isolated from Pinctada fucata Mucus[J]. FOOD SCIENCE, 2012, 33(21): 217-220.

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