Antifungal Activity of Marine Pseudomonas GY-1 and Purification of the Antifungal Protein

Abstract

Abstract: In the present study, agar-well diffusion method and Oxford Cup method were used to detect the antimicrobial activities of marine Pseudomonas GY-1 strain. The results showed that GY-1 strain and its fermentation broth had relatively strong inhibition activity against a variety of phytopathogenic fungi, including Bipolaris sorokiniana, Fusarium graminearum, Alternaria solania, Alternaria alternate, Sclerotinia sclerotiorum and Alternaria tenuis Nees. Further study had successfully purified a protein from Pseudomonas GY-1 by ammonium sulfate fractional precipitation, DEAE sepharose fast flow ion exchange chromatography and Sephadex G-75 filtration chromatography. This protein showed obvious inhibition activity against B. sorokiniana. SDS-PAGE analysis revealed that the molecular weight of the protein was about 70.9 kD.

Key words: marine Pseudomonas GY-1, antifungal activity, antifungal protein, isolation and purification

CLC Number:

Q939.96

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