Optimizing Conditions for Enzymatic Extraction of Flavonoids from Lotus Leaves

Abstract

Abstract: This study aimed at determining the optimum hydrolysis conditions for the extraction of flavonoids from lotus leaves. Cellulase and pectinase were used in combination for one-step hydrolysis of lotus leaves and the solvent used was 70%. The effects of five extraction conditions including solid-to-solvent ratio, pH, enzyme dosage, temperature and hydrolysis time on extraction efficiency were investigated by one-factor-at-a-time method. The optimum solid-to-solvent ratio was found to be 1:20 g/mL. pH, enzyme dosage, temperature and hydrolysis time were optimized using a 16-run orthogonal array design to be 3.5, 3.0 h, 35 ℃, 2800 U/g cellulase + 2000 U/g pectinase, respectively. Under the optimized conditions, the extraction yield of flavonoids was 3.74% compared with 1.59% for aqueous extraction technique as optimized also using a 16-run orthogonal array design (P＜0.01). Hence, we concluded that enzymatic hydrolysis provides a high-yield method for the extraction of flavonoids from lotus leaves.

Key words: flavonoid, lotus leaf, enzymatic hydrolysis, extraction efficiency

CLC Number:

R248.2

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