Rapid HPLC Determination of Angiotensin Ⅰ-Converting Enzyme Inhibitory Activity

Abstract

Abstract: A rapid HPLC method was developed for the determination of angiotensin Ⅰ-converting enzyme (ACE) inhibitory activity. Hippuric acid was separated on an ODS C18 column (150 mm × 4.6 mm, 5 μm) using a mobile phase consisting of acetonitrile and water (20:80, V/V, containing 0.5‰ methanoic acid) at a flow rate of 1 mL/min and detected at 228 nm. A good linear relationship was observed for hippuric acid over the concentration range of 0.1 to 500 μg/mL with a correlation coefficient of 0.9999. The average spike recovery rates of hippuric acid were 89.77%–99.57% with a relative standard deviation (RSD) of 0.01%–1.52% (n = 3). The HPLC method was simple, rapid, reproducible, accurate and suitable for the analysis of ACE inhibitory activity in vitro as demonstrated by its applications to determine different ACE inhibitors.

Key words: high performance liquid chromatography (HPLC), angiotensin I-converting enzyme (ACE), hippuric acid (HA)

CLC Number:

TS207.3

References

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