A Real-Time PCR Method for Detection of Cordyceps sinensis (Berk.) Sacc.

Abstract

Abstract: An efficient and sensitive method was established to detect Cordyceps sinensis (Berk.) Saccardo using real-time PCR. The internal transcribed spacer (ITS) region of ribosomal DNA (rDNA) in Cordyceps sinensis (Berk.) Saccardo. was used as a target molecular marker and sequence homology analysis was carried out on various Cordyceps species. A set of specific primer and probe for real-time PCR was designed which allowed the amplification of 80-bp DNA fragments. The detectable DNA template concentration of the real-time PCR method for Cordyceps sinensis (Berk.) Saccardo was as low as 0.0001 ng/μL.

Key words: Cordyceps sinensis, real-time PCR, identification

CLC Number:

Q939.99

References

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