Microwave-Assisted Extraction and Composition Analysis of Flavonoids from Perilla frutescens Leaves

Abstract

Abstract: Response surface methodology was used to optimize conditions for the microwave-assisted extraction of flavonoids from Perilla frutescens leaves. The effects of raw material particle size, extraction time, temperature, ethanol concentration and solid-to-solvent ratio on extraction efficiency were investigated. The optimal conditions for extracting flavonoids from Perilla frutescens leaves were found to be extraction at 71 ℃ for 70 min with 61% ethanol at a solid-to-solvent ratio of 1:20 (g/mL) and a raw material particle size of 20 mesh. Under these conditions, the maximum extraction yield of flavonoids was 59.28 mg/g. The obtained extract was purified by D152 macroporous resin column chromatography and the purified product mainly consisted of lutin and luteolin as demonstrated by UV absorption spectrometry and HPLC.

Key words: microwave, Perilla frutescens, flavonoids, response surface methodology, column chromatography, full wavelength scan, high performance liquid chromatography (HPLC)

CLC Number:

TS202.1

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