Extraction and Antioxidant Activity of Polysaccharides from Immature Fruits of Schisandra chinensis (Turcz.) Baill

Abstract

Abstract: The extraction of water-soluble polysaccharides from immature fruits of Schisandra chinensis (Turcz.) Baill was optimized by one-factor-at-a-time and orthogonal array design methods. Crude polysaccharides were separated from the resulting extract by means of ethanol precipitation and deproteinized by Sevag method. The antioxidant activity of crude and purified polysaccharides was investigated and compared with that of VC. The optimum extraction conditions were found to be three cycles of extraction at 90 ℃ for 2 h with a solid-to-liquid ratio of 1:30 (g/mL). Purified polysaccharides contained nearly no protein or nucleic acid as demonstrated by UV spectroscopic analysis and had common spectral characteristics of polysaccharides as showed by infrared spectroscopic analysis. Both polysaccharide samples had scavenging effects against hydroxyl and superoxide anion free radicals and the free radical scavenging effect of deproteinized polysaccharides was stronger. The corresponding IC50 values against superoxide anion and hydroxyl free radicals were 66.03 mg/mL and 6.49 mg/mL, respectively, suggesting purified polysaccharides to be a strong scavenger against hydroxyl free radicals. Crude and deproteinized polysaccharides, however, had weaker antioxidant activity than VC.

Key words: immature fruits of Schisandra chinensis (Turcz.) Baill, polysaccharides, extraction, antioxidant activity

References

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