Abstract: The present study was conducted to determine optimal conditions for the purification of salicin from the dried roots of Alangium chinense (Lour.) by macroporous resin adsorption. Among 8 types of macroporous resins, HPD-826 was found to be the best one for the adsorption and desorption of salicin. Adsorption and desorption conditions were optimized for the application of HPD-826 to purify salicin from crude extract from the dried roots of Alangium chinense (Lour.). The best purification results were obtained when 6.5 BV of crude extract sample at a concentration 45.12 μg/mL was loaded onto an HPD-826 column with a diameter/height ratio of 1:8 at a flow rate of 3 BV/h and afterwards, the column was washed with 4 BV of deionized water for the removal of water-soluble impurities and eluted with 5 BV of 30% ethanol. The resulting recovery of salicin was approximately 78%. In conclusion, HPD-826 had a good overall performance for purifying salicin, and the proposed procedure was stable and feasible and therefore suitable for industrial applications.

Key words: Alangium chinense (Lour.), salicin, macroporous adsorption resin, purification