Abstract: A high-yield method for the extraction of polysaccharides from Sargassum pallidum was proposed using an integrated ultrasonic and microwave reaction system. The extraction yield of polysaccharide was measured by phenol-sulferic acid method. The optimal conditions of raw material particle size, extraction time, solid-to-solvent ratio, ultrasonic power and microwave power for extracting polysaccharides from Sargassum pallidum were 40 mesh, 20 min, 1:25 (g/mL), 75% and 200 W, respectively, as determined by orthogonal array design. Among five operating parameters, raw material particle size was the most important factor for polysaccharide extraction. The combined use of ultrasonic and microwave provided a time-saving and high-yield method for polysaccharide extraction from Sargassum pallidum.

Key words: ultrasonic-microwave, polysaccharide, extraction process, Sargassum pallidum