Abstract:

[Abtract] Objective: To observe the inhibition of the deuterium-depleted-alcohol on the proliferation of lung cancer cell A549 and H460. Methods: In vitro, the inhibition effects of alcohol, deuterium-depleted water (DDW) and deuterium-depleted-alcohol (DDA) on the proliferation of lung cancer cell A549 were measured by MTT assay, and the apoptosis were determined by TUNEL method and flow cytometry. In vivo, human lung cancer H460 cells were inoculated intradermally into nude mice. After 9 days, the growth curve was drawn to show the growing characteristics of xenograft. After 21 days, we sacrificed the mice, weighed and calculated the ratios of tumor-suppression. We also detected the pathological changes of tumor tissues to observe the tumor necrosis degree by HE staining. The pathological changes of the liver and lung tissues were also observed. Results: The proliferation ability of A549 was remarkably inhibited at the concentration of 180 mM of DDA, compared with the control group and the alcohol group(p <0.05); Apoptosis of A549 was remarkably induced by DDA, compared with the control group、the alcohol group and DDW group(p <0.05); Moreover, FCM assay results showed that DDA could promote apoptosis. The in vivo data showed the tumor inhibition rates were higher in DDA and DDW group than that in the control group (p <0.05), 31.014% and 27.639% respectively. HE staining showed that tumor cells necrosed and arranged loosely in DDA and DDW group, and no obvious changes in lung and liver tissue had been observed. Conclusion: DDA has evident inhibition effect on the proliferation of lung cancer cell.