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Optimization of Corn Protein Hydrolysis by Neutrase and Influence of Hydrolysates on the Activity of Alcohol Dehydrogenase

GUO Qing-qi,ZHANG Nai-xun,WANG Qun,WANG Sha,HE Fu-gui   

  1. School of Forestry, Northeast Forestry University, Harbin 150040, China
  • Online:2013-07-15 Published:2013-06-28
  • Contact: GUO Qing-qi

Abstract:

Protein hydrolysis of defatted corn gluten by neutrase was optimized using response surface methodology in this
study. Results showed that the best hydrolysis conditions were found to be 3.4 h, 1.25%, 6.95 and 1:23 (m/V) for hydrolysis
time, enzyme/substrate ratio, pH and material/liquid ratio, respectively. Under these conditions, the predicted and actual
values of degree of hydrolysis (DH) were 16.51% and (16.38 ± 0.11)%, respectively. The corn protein hydrolysate prepared
under the optimal conditions activated alcohol dehydrogenase by (21.23 ± 0.17)%. Amino acid analysis showed higher
contents of alanine and leucine in the hydrolysate.

Key words: corn proteins, neutrase, hydrolysis, response surface methodology, alcohol dehydrogenase

CLC Number: