Study on antioxidant activity of four kind of grape

Abstract

Abstract: In this paper,by measuring the total antioxidant capacity, scavenging DPPH ? ability,scavenging ? OH and O2-? ability to evaluate antioxidant capacity of the three extract of four grape juice in vitro. By animal experiments to evaluate antioxidant capacity of the AE extracting from the Muscat grape juice in vivo ,and by chemical qualitative and quantitative analysis the active ingredient of the AE from the Muscat grape juice. The results showed that: there were significantly difference among the same extracts of different kind of grape juice on antioxidant activity aspect in vitro. The vitro antioxidant capacity of the AE from the Muscat grape juice was strongest. The AE [800mg / (kg bw ? d) ] from the Muscat grape juice could increase serum and liver tissue SOD activity ,and reduce the MDA content in liver tissue. The AE from the Muscat grape juice contains polysaccharides, organic acids, flavonoids, polyphenols and other antioxidant substances,which have total phenolics 0.456mg/ml ,total flavonoids4.37 mg/ml.

CLC Number:

TS201.2

References

[1] Lv YZ， Song Y， Wu GH.The antioxidant activity of grape polyphenols[J].Food Sci， 2006，27(12)：213.
[2] Bagchi D， Bagchi M，Stohs SJ，et al.Free radicals and grape seed proanthocyanidin extract：importance in human health and discase prevention[J].Toxicology， 2000，148(2-3)：187-197
[3] Huang S S，Tsai M C，chih C L，et al.Resveratrol reduction of infarct size in longevans mice subjected to focal cerebral ischemia[J].Life Sci， 2001，69(9)：1057-1065.
[4] Jian M.Active oxygen and its biological function[J].Progress in biochemistry and biophysics，1981，38(2)：23-29
[5] Valko M，Leibfritz D，Moncol J，Cronin M T D，Mazur M，&Telser J. Free radical and antioxidant in normal physiological functions and human disease[J]．Int J Biochem Cell B，2007，39：44-84
[6]Perry G，Raine K.A.，NunomuraA.，Watayc T.，Sayre M.M.，＆Smith M.A.How important is oxidative damage?Lesson from Alzheimer’S desease[J].Free Radical Biol.Med，2000，22：831-834
[7]Kamat J.P.，Devasagayam T.P.A.Oxidative damage mitochondria in normal and cancer tissues，and its modulation[J].Toxicology，2000，155：73-82
[8]Minussi R．C．，Rossi M．，Bologna L．，Cordi L．，Rotilio D．，Pastore GM．，&Duran N．Phenolic compounds and total antioxidant potential of commercial wines[J]．Food Chem，2003，82：409-416
[9]IQBALS，HALEEMAS，AKHTARM，et al.Efficiency of pomegranate peel extracts in stabilization of sunflower oil under accelerated conditions[J].Food Reasearch Internation，2008，41：194-200
[10]BAESH，SUHHJ.Antioxidant activities of five different mulberry cultivars in Koreal[J].Food Science and Technology，2007，40(6)：955-962
[11] Bagchi D，Garg A，Krohn RL，et al.Oxygen freen radical scavenging abilities of vitamins C and E，and a grape seed proanthocyanidin extract in vitro[J].Res Commun Mol Pahol pharmacol，1997，95(2)：179-189
[12]Castillo J，Benavente Garcia O，Lorente J，et al.Antioxidant activity and radioprotective effects against chromosomal damage induced in vivo by X-rays of flavan-3-ols(procyanidins) from grape seeds(Vitis vinifera)：comparative study versus other phenolic and organic compounds[J].J Agric Food Chem，200，48(5)：1738-1745
[13]Chanvitayapongs S，Draczynka-Lusiak B，Sun AY.Amelioration of oxidative stress by antioxidants and resveratrol in PCl2 cells [J].Neuro report，1997，8(6)：1499-1502
[14] Lv LS， Cao D.Extract oligomeric procyanidins of defatted grape seed[J]. Journal of wuxi university of light industry，2001，20(2)：208-210
[15] Peng CL， Chen SW， Lin ZF.Detection of Antioxidative Capacity in Plants by Scavenging Organic Free Radical DPPH[J].Progress in biochemistry and biophysics，2000，27(6)：658-661
[16] Li Y， Bao HY.Study on lipid oxidation and antioxidation[J].China food additives，1997，(4)：4-9.
[17] Cheng SJ，Jiang S.Phenolic Compounds and Their Antioxidant Activity in Fruits and Vegertables[J].Journal of Anhui Technical Teachers College，1997，17(2)：144-148
[18] Kelley MiHeazdina G.Interaction of nitrogen availability duaring bloom and light intensity during veraison[J].American Journal of Enology and Viticulture，1998，49(3)：341-349
[19] Sheng W.Study on Antioxidant Activity of Extracts from Bulbil of Dioscorea opposita[J].Food Sci，2009，3(30)：92-93.
[20] Lian XJ.Study on antioxidant activity of resistant starch of differents weet potatoes by DPPH method[J].Food stuff and fats，2009，6：26-28.
[21] Zhao YH， Li JK， Zhao W.Evaluation on Antioxidant Activities of Extracts from Common Edible and Medicinal Plants in vitro[J].Food Sci，2009，3(30)：104-106
[22] Beijing University of Chinese Medicine.Chemistry of Chinese materia medica[M].Shang Hai：ShangHai people's publishing office，1976
[23] Li J， Nie JY， Li HF.Folin-Ciocalteus Determination the total polyphenols of grape and wine[J].China Southern Fruit，2007，36(6)：16-23.
[24] Jing SQ， zheng L， Su J.Extraction and determination of total flavonoids and polysaccharides from persistent calyx of physalis alkekengil[J].Food research and development，2008，29(6)：82-84.
[25] Li JH， Ma HQ， Chen SW.Stusies on antimicrobial effect of Grapepolyphenols[J].Journal of Chinese Institute of Food Science and Technology，2008，8(4)：100-101.