FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (3): 141-146.

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Chemical structure character of a purified polysaccharide from Leaves of Camellia chrysantha (Hu) Tuyama

  

  • Received:2012-08-20 Revised:2013-01-12 Online:2013-02-15 Published:2017-12-29
  • Contact: Hua-Juan LIN E-mail:huajuanlin@vip.163.com

Abstract: Hypolipidaemic active was verified to be one of bioactive functions for crude polysaccharide from Camellia chrysantha (Hu) Tuyama, however, little is known about their chemical character. In this paper, a purified polysaccharide (Named TPS3-1) with molecular weight of 4.15×106u was obtained by ion-exchange chromatography and gel filtration chromatography, and the chemical character of its hairy region was studied by analysis of sugar composition, infrared spectrum (IR), nuclear magnetic resonance (NMR) and partially acidic hydrolysis by steps. The result of sugar composition analysis showed that the content of neutral sugar was about 2 fold to that of galacturonic acid (GalA), in which the ratio of rhamnose (Rha), alabinose (Ala) and galactose (Gal) was 1:4.2:3.0:0.4. In addition, 62.58% of GalAs existed as methyl esterified form. The result of partially acidic hydrolysis showed that approximately 73% of the neutral sugars were released to the solution of 0.01 Mol/L trifluoroacetic acid (TFA), and the molecular fragment was suggested to be consisted of branch chains including glucan, alabinan and galactan. 5% of the neutral sugars were released to the solution of 0.05 Mol/L TFA, which molecular fragment was suggested to be some oligosaccharides as branch chains, where alabinose may be alternately connected with glucose or galactose. 13% of the neutral sugars were released to the solution of 0.5 Mol/L TFA, which molecular fragment was suggested to be some oligosaccharides as branch chains, where some alabinoses may be alternately connected to galactose, and some mono alabinoses scarcely attached to the homogalacturonan bond chain.