FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (3): 184-187.

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Preparation and Identification of Anti-idiotypic Antibody against Anti-Zearalenone Monoclonal Antibody

  

  • Received:2011-11-17 Revised:2012-12-29 Online:2013-02-15 Published:2017-12-29

Abstract: Abstract:Purpose:To lay the foundation for develop the toxin-free detection method for zearalenone(ZEN), an anti-idiotypic antibody(AId) against anti-zearalenone toxin monoclonal antibody(McAb) was prepared.Methods:The polyclonal anti-idiotype antibody against anti-ZEN McAb was prepared by immunizing BALB/c mouse with the conjugate McAb - KLH ,and the AId was detected by ELISA with Fab fragment as coating antigen.Results:The obtained AId against anti-ZEN McAb was identified as the“inner image”with ZEN toxin,and the correlation coefficient reached to 0.99.Conclusion:The polyclonal AId against anti-ZEN McAb was prepared successfully and could be used to replace ZEN toxin standard in ELISA. 

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