Preliminary Study on Lipoxygenase Acitivity of Fresh Corn

Abstract

Abstract: The affecting factors of fresh corn lipoxygenase(LOX) were studied using spectrophotometric assay. With linoleic acid as substrate, and fresh sweet corn and waxy corn as samples, we determined the LOX activity changes in the reaction system with different extraction positions, enzymatic reaction pH values and temperatures, and substrate concentrations. The results indicate that LOX activity in the germ is significantly higher than in the whole kernel, and the enzyme has the optimal temperature of 55℃, the optimal pH of 6.0, and the optimal substrate concentration of 10.0mmol/L. The thermal stability tests demonstrate that LOX activity is effectively inhibited by increasing temperature and prolonging heat treatment.

CLC Number:

TS201.2

References

[1] Theerakulkait C, Barrett D M, Mcdaniel M R. Sweet corn germ enzymes affect odor formation[J]. Journal of Food Science, 1995, 60(5): 1034-1040.
[2] Rodriguez-saona L E, Barrett D M, Selivonchick P. Peroxidase and lipoxygenase influence on stability of polyunsaturated fatty acids in sweet corn (Zea mays L.) during frozen storage[J]. Journal of Food Science, 1995, 60(5): 1041-1044.
[3] 胡廷章, 胡宗利, 屈霄霄, 等. 植物脂肪氧化酶的研究进展[J]. 生物工程学报, 2009, 25(1): 1-9.
[4] Surrey K. Spectrophotometric method for determination of lipoxidase activity[J]. Plant Physiol, 1964, 39(1): 65-70.
[5] Reyes-de-corcuera J I, Cavalieri R P. Improved amperometric method for the rapid and quantitative measurement of lipoxygenase activity in vegetable tissue crude homogenates[J]. Journal of Agricultural and Food Chemistry, 2002, 50(5): 997-1001.
[6] 李会容, 赵昶灵, 杨焕文, 等. 高等植物脂氧合酶活性测定方法的研究进展[J]. 中国农学通报, 2011, 27(15): 217-223.
[7] 钟芳, 王璋, 许时婴. 3种脂肪氧合酶酶活测定方法[J]. 无锡轻工大学学报, 2001, 20(1): 77-80.
[8] Axelrod B, Cheesbrough T M, Laakso S. Lipoxygenase from soybeans[J]. Methods in Enzymology, 1981, 71: 441-451.
[9] 陈毓荃. 生物化学实验方法和技术[M]. 北京:科学出版社, 2002: 218-222.
[10] Theerakulkait C, Barrett D M. Lipoxygenase in sweet corn germ: isolation and physicochemical properties[J]. Journal of Food Science, 1995, 60(5): 1029-1033.
[11] 汪仁, 沈文飚, 翟虎渠, 等. 植物种子脂氧合酶[J]. 植物生理学通讯, 2005, 41(3): 388-394.
[12] Romero M V, Barrett D M. Rapid methods for lipoxygenase assay in sweet corn[J]. Journal of Food Science, 1997, 62(4): 696-700.
[13] Mahanta P K, Tamuli P, Bhuyan L P. Changes of fatty acid contents, Lipoxygenase activities, and volatiles during black tea manufacture[J]. Journal of Agricultural and Food Chemistry, 1993, 41(10): 1677-1683.
[14] 李强, 吴晓露, 李红伟, 等. 黄瓜果实脂氧合酶活性测定方法的建立[J]. 东北农业大学学报, 2008, 39(5): 62-65.
[15] Perraud X, Kermasha S, Bisakowski B. Characterization of a lipoxygenase extract from Geotrichum candidum[J]. Process Biochemistry, 1999, 34(8): 819-827.
[16] 陈石根, 周濡琦. 酶学[M]. 上海:复旦大学出版社, 2001: 199-203.
[17] Shiiba K, Negishi Y, Okada K, et al. Purification and characterization of lipoxygenase isozymes from wheat germ[J]. Cereal Chemistry, 1991, 68(2): 115-122.
[18] Kuo J, Hwang A, Yeh D, et al. Lipoxygenase from banana leaf: Purification and characterization of an enzyme that catalyzes linoleic acid oxygenation at the 9-position[J]. Journal of Agricultural and Food Chemistry. 2006, 54(8): 3151-3156.
[19] 李铭韧, 史国安. 牡丹花瓣脂氧合酶测定方法的研究[J]. 山东农业科学, 2010, 9: 91-93.
[20] Bsysal T, Demird?venb A. Lipoxygenase in fruits and vegetables: A review[J]. Enzyme and Microbial Technology, 2007, 40(4): 491-496.