FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (3): 228-232.

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Preparation Antihypertensive Peptides from Rapeseed Protein by Double Enzymes

  

  • Received:2011-10-25 Revised:2012-12-26 Online:2013-02-15 Published:2017-12-29

Abstract: In this paper,rapeseed proteins were hydrolyzed by two different enzymes to prepare antihypertensive peptides. Alcalase and Neutral protease were selected to prepare antihypertensive peptides. Angiotensin-converting enzyme inhibitory activity (ACEIA) is analyzed by UV spectrophotometer. The effects of three crucial factors on the ACEIA of rapeseed proteins were analyzed by single-factor method, Subsequently, Box-Behnken experimental design combining with response surface methodology and quadratic programming was employed for maximizing the Alcalase-catalyzed hydrolysis of rapeseed proteins and a quadratic regression model was obtained using the Design-Expert software. the determination of optimum conditions of the rapeseed protein hydrolization was performed and the results listed as follows: substrate concentration 5.65 %, ratio of enzyme to substrate ([E]/ [S]) 6.5%,temperature 54.9 ℃, The theoretical value of ACEIA is 50.303%,and the actual value is 50.12%.

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