Preparation and monosaccharide composing analysis of homogeneous polysaccharide FVP60-C2 from the fruiting bodies of Flammulina velutipes

Abstract

Abstract: The polysaccharide was extracted from fruiting bodies of Flammulina velutipes by hot water extraction, ethanol precipitation. The crude polysaccharide was purified to obtain FVP60-C2 by Ion exchange column chromatography and Gel-filtration column chromatography. The physicochemical properties of FVP60-C2 were investigated by HPLC, UV and IR. Following the hydrolysis and acetylation of FVP60-C2, the monosaccharide composition of FVP60-C2 was analyzed by GC. The results showed a homogeneous polysaccharide FVP60-C2, with molecular weight 1.429×104 Da, was obtained, which is consist of fucose, xylose, mannose, glucose and galactose with the molar ratio of 0.74:0.21:1.36:1.00:1.31.

CLC Number:

TS201.1

References

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