FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (4): 154-156.

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The fast qualitative analysis and accurate quantitative analysis of neotame in wine by high performance liquid chromatography method

  

  • Received:2012-02-07 Revised:2013-01-16 Online:2013-02-25 Published:2013-01-29

Abstract:

A high performance liquid chromatography (HPLC) method for the fast qualitative analysis and accurate quantitative analysis of the artificial sweetener neotame in wines was developed. Neotame was fast qualitative analysis on a Waters Nova-Pak C18 column with an ultraviolet detector at 218 nm using acetonitrile: ion-pair reagent=40:60 (V/V) as mobile phase, and the retention time of neotame was about 13.4 min. The contents of neotame in the suspected samples were determined under gradient elution condition, and the retention time of neotame was about 15.9 min. The detection limit of the developed method was 0.37 μg/mL, and the mean spike recoveries for neotame in a blank wine sample were between 99%~101% , with a relative standard deviation below 1.0% (n=5). The method proved to be simple, accurate and practical, and it can save 50% time in analysis of large quantities of samples.

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