Abstract: Abstract: The polyphenols was extracted with ethanol from blueberry leaves. According to the experiments of single factors, Plackett Burman design was applied to study the factors of extraction and it was found that ethanol concentration (p=0.0025), extraction temperature (p=0.0091), solid-liquid ratio (p=0.0236) were the key factors to the extraction. It was optimized on the extraction of polyphenols from blueberry leaves by response surface method. A quadratic equation model for phenolics extraction was set up and the effects of the few factors and their corresponding relationships was obtained. The optimal extraction conditions was as follows: ethanol concentration 62.05%, extraction temperature 67.54℃, solid-liquid ratio 1:23.65, extraction time 2.06h. Under the optimized condition, the extraction yield was 91,02%. The model played an important role in predicting the extraction of polyphenols from blueberry leaves.