Abstract: Purpose: To establish a method for the separation and preparation of galangin from Alpinia officinarum Hance. Methods: Two methods were developed for the preparative separation of galangin from Alpinia officinarum Hance, counter-current chromatography (CCC) directly and counter-current chromatography (CCC) combined with silica gel column chromatography. Results: The crude extract was pre-separated by silica gel chromatography, then separated by CCC with a two-phase solvent system composed of petroleum ether-ethyl acetate-ethanol-water (2:0.4:0.8:0.9, v/v) and high purity of galangin was obtained. 15.6mg galangin with the purity of 99.1% and 5.2mg unknown compound Ⅱ with the purity of 95.2% were obtained. Conclusions: The result indicates that silica gel chromatography combined with counter-current chromatography(CCC) can separate and preparate high purity of galangin from Alpinia officinarum Hance. This method has practical value and provides theoretical foundation for the further development．The method can be used for the preparation of the relevant standard materials and the development of functional factors.