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Isolation and Identification of a Di-n-butyl phthalate (DBP)-Degrading Strain H-2 and Its Degradation Characteristics

JIN Lei, CHEN Yu, YAN Zhong-yong, GUO Yuan-ming, ZHU Yin, LONG Ju, GU Bei-qiao   

  1. 1. Marine Fishery Research Institute of Zhejiang Province, Zhoushan 316100, China;
    2. Zhejiang Province Key Laboratory of Mariculture and Enhancement, Zhoushan 316100, China
  • Online:2014-08-15 Published:2014-08-25

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Abstract:

A broad-spectrum and efficient di-n-butyl phthalate (DBP)-degrading bacterial strain H-2 was isolated from
plastic food packaging garbage-contaminated soil by enrichment culture and purification. Based on its morphological,
physiobiochemical characteristicsand 16S rRNA gene sequence, strain H-2 was identified as Paenibacillus sp. By high
performance liquid chromatography (HPLC), its DBP degrading characteristics and diversity of degradable substrates
were studied. The results showed that the optimal temperature and pH for DBP degradation by strain H-2 was 30 ℃ and
7.0, respectively. Under these conditions, H-2 degraded more than 87.6% of 100 mg/L DBP within 3 d. The diversity of
degradable substrates showed that strain H-2 could degrade phthalate (DMP), DEP and DBP efficiently, while DOP was
degraded difficultly by strain H-2. This research may provide the theoretical basis to decontaminate PAEs pollution of plastic
food packaging garbage.

Key words: di-n-butyl phthalate, Paenibacillus sp., degradation characteristics, degradation spectrum

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