Abstract:

An ultra performance liquid chromatography (UPLC) method was established for the determination of boscalid
and pyraclostrobin in mango. Samples were extracted with acetonitrile, purified on NH2 column, and analyzed by gradient
elution using acetonitrile-water as the mobile phase. The residues were determined by dual-wavelength ultraviolet detector,
and quantified by an external standard method. An excellent linearity for boscalid and pyraclostrobin was observed in the
range of 0.10–4.00 μg/mL and 0.02–1.00 μg/mL with correlation coefficients higher than 0.999, respectively. The limit of
detection for both pesticides was 0.002 mg/kg. The average recovery rate for the pesticides from mongo at three spiked
concentrations was in the range of 76.00%–103.00% with relative standard deviations of 1.07%–13.28%.

Key words: ultra performance liquid chromatography (UPLC), mango, boscalid, pyraclostrobin