Effect of Temperature on Muscle Nutritional Components and Blood Biochemical Parameters of
Pelodiscus sinensis Alive without Water

doi:10.7506/spkx1002-6630-201406042

Abstract

Abstract:

Pelodiscus sinensis was kept alive without water by incubation in a thermostatic chamber set at different
temperatures (4, 10 and 18 ℃) for 21 days. The changes in body weight loss, the contents of water, lipid and protein in
muscle, and the concentrations of glucose (Glu), total protein (TP), albumin (ALB), urea, uric acid (UA) and creatinine
(CREA) as well as the activities of lactate dehydrogenase (LDH), alkaline phosphatase (AL P) and aspartate aminotransferase
(AST) in blood were examined during the incubation period. The results indicated that the survival time of Pelodiscus
sinensis at 4 ℃ was 11–16 days. The body weight loss and nutrient consumption of Pelodiscus sinensis at this temperature
were lower than those observed at 10 and 18 ℃ (P ＜ 0.05) and the concentrations of Glu, UA and CREA , and the
activities of LDH and AST were also higher (P ＜ 0.05). The body weight loss, nutrient consumption and blood urea
concentration of Pelodiscus sinensis at 18 ℃ were higher than those observed at 4 and 10 ℃ (P ＜ 0.05). Additionally, the
nutrient consumption and blood physiological indexes of Pelodiscus sinensis at 10 ℃ were between those at 4 and 18 ℃.
The nutrition, blood TP and ALB levels and ALP activity of Pelodiscus sinensis at three different temperatures showed a
decreasing trend during the incubation period, while the body weight loss, and urea and UA levels exhibited an increasing
trend. The levels of ATP, LD H, AST and CREA in Pelodiscus sinensis at 4 ℃ increased initially and then decreased.
Furthermore, ATP content of Pelodiscus sinensis at 10 and 18 ℃ exhibited an initial decrease followed by a increase,
while AST activity always decreased. These results suggest that the nutrient consumption of Pelodiscus sinensis at 4 ℃ is
lower, while low temperature stress has a harmful effect on the metabolism and vitality of Pelodiscus sinensis. The body
metabolism rate of Pelodiscus sinensis at 18 ℃ is faster and the nutrient consumption and body weight rate are higher.
Therefore, the most appropriate temperature for keeping Pelodiscus sinensis alive is about 10 ℃.

Key words: Pelodiscus sinensis, temperature, nutrition, biochemical parameters

CLC Number:

TS254.4

HE Rong, XIE Jing*, SU Hui, LI Liu, HUANG Shuo-lin. Effect of Temperature on Muscle Nutritional Components and Blood Biochemical Parameters of
Pelodiscus sinensis Alive without Water[J]. FOOD SCIENCE, 2014, 35(6): 194-199.

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